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KMID : 0545120010110030491
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Journal of Microbiology and Biotechnology
2001 Volume.11 No. 3 p.491 ~ p.496
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Improvement of Decarboxylating Agar Medium for Screening Biogenic Amine-Producing Bacteria in Kimchi
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Mah, Jae Hyung
Shin, Soon Young/Lee, Heung Shick/Cho, Hong Yon/Hwang, Han Joon
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Abstract
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A modification of decarboxylating agar medium as described by Niven was performed to improve the detection method of biogenic amine-producing bacteria and to eliminate the false-positive. A total of 120bacterial strains isolated from kimchi were used to evaluate different decarboxylating agar media and for screening biogenic amines. Potential false-positives ranged from approximately 66 to 79% of the strains tested in the already well-known media. In our improved medium, none of the 120strains showed the potential false-positives. There was a good agreement (81.7% -87.5%) between the results obtained by the improved medium and by HPLC analysis. Consequently, this medium was greatly improved in screening biogenic amine-producing bacteria and discarding false-positives. Of the 120 kimchi isolates, 14.2, 18.3, 37.5, and 0.8% were found by HPLC to be the producers of histamine, tyramine, putrescine (as a form of spermine), and cadaverine, respectively. The proportion of biogenic amine producer during kimchi fermentation increased to a maximum at an immature period and decreased thereafter.
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